# 160 pick_variant

1. Func.refGene   包含 ~/exonic/ 不包含 ~/ncRNA/

2. ExonicFunc.refGene 不等于 "synonymous SNV" "unknown"

3. 1000g2015aug_all 1000g2015aug_eas esp6500siv2_all ExAC_nontcga_ALL ExAC_nontcga_EAS gnomAD_genome_ALL gnomAD_genome_EAS  < 0.01

4. Otherinfo10 eq 'PASS'

5. ```
   ($line[$#line]!~/STR/ or (length($line[3])>=4 or length($line[4])>=4) or
          $FREQ>5)
   ```

   最后一列没有STR 

   Ref Alt 长度大于4 频率大于5%

# 17

1.