diff --git a/tools/t7.py b/tools/t7.py
index dafe016..a6f4d9c 100644
--- a/tools/t7.py
+++ b/tools/t7.py
@@ -247,7 +247,9 @@ class AutoLayout:
         notrepeatbarcode = True
         if self.chip_barcode_recode[chipname].intersection({item['barcode'] for item in library_data['data']}) or \
                 self.chip_barcode_recode[chipname].intersection(
-                    {'N' * 8 + item['i7'] for item in library_data['data']}):
+                    {'N' * 8 + item['i7'] for item in library_data['data']}) or \
+                self.chip_barcode_recode[chipname].intersection(
+                    {item['i5'] + 'N' * 8 for item in library_data['data']}):
             notrepeatbarcode = False
 
         # 互斥的文库